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Stimulation of die SSS results in expression of Fos-like immunoreactivity in restricted regions of the caudal medulla and high cervical spinal cord as has been previously reported from our group Kaube et al., 1993a ; . The data here extend the number of animals that we have studied n 3 ; at rate of stimulation of 0.3 Hz. The regions expressing Fos in the trigeminal system are the ventrolateral portion of laminae I and IIo of die trigeminaJ nucleus caudalis and Q dorsal horn grey matter and the medial aspect of laminae I and Ho of the C2 dorsal horn grey matter. In addition to these cells Fos was also detected in the caudal portion of the medial nucleus of the solitary tract and in the nucleus retroambigualis at the level of die trigeminal nucleus caudalis and in laminae X in die high cervical spinal cord. The quantitative data for these regions are presented in Table 2.
To determine the inhibitory profile of the novel somatostatin SRIF ; analog SOM230 with broad SRIF receptor binding, we compared the in vitro effects of SOM230, octreotide OCT ; , and SRIF-14 on hormone release by cultures of different types of secreting pituitary adenomas. OCT 10 nM ; significantly inhibited GH release in seven of nine GH-secreting pituitary adenoma cultures range, 26 to 73% ; , SOM230 10 nM ; in eight of nine cultures range, 22 to 68% ; , and SRIF-14 10 nM ; in six of six cultures range, 30 to 75% ; . The sst analysis showed predominant but variable levels of somatostatin receptor sst ; 2 and sst5 mRNA expression. In one culture completely resistant to OCT, SOM230 and SRIF-14 significantly inhibited GH release in a dose-dependent manner with an IC50 value in the low nanomolar range. In the other cultures, SOM230 showed a lower potency of GH release inhibition IC50, 0.5 nM ; , compared with OCT IC50, 0.02 nM ; and SRIF-14 IC50, 0.02 nM ; . A positive correlation was found between sst2 but not sst5 mRNA levels in the adenoma cells and the inhibitory potency of OCT on GH release in vivo and in vitro, and the effects of SOM230 and SRIF-14 in vitro. In three prolactinoma cultures, 10 nM OCT weakly inhibited prolactin PRL ; release in only one 28% ; , whereas 10 nM SOM230 significantly inhibited PRL release in three of three cultures 23, 51, and 64.0% ; . The inhibition of PRL release by SOM230 was related to the expression level of sst5 but not sst2 mRNA. Several conclusions were reached. First, SOM230 has a broad profile of inhibition of tumoral pituitary hormone release in the low nanomolar range, probably mediated via both sst2 and sst5 receptors. The higher number of responders of GHsecreting pituitary adenoma cultures to SOM230, compared with OCT, suggest that SOM230 has the potency to increase the number of acromegalic patients which can be biochemically controlled. Second, compared with OCT, SOM230 is more potent in inhibiting PRL release by mixed GH PRL-secreting adenoma and prolactinoma cells. J Clin Endocrinol Metab 89: 15771585, 2004.
We sponsor a savings plan which provides benefits to substantially all U.S. associates and under which we match employee contributions at 50% of the first 6% of total pay, which includes base salary, overtime and annual performance-based bonuses. Vesting of the Company match occurs after three years for new employees and is 100% for all employees hired before January 1, 1997. The expense in fiscal years 2000, 1999 and 1998 for the match was .6 million, .5 million and .1 million, respectively. Under the plan, we also have the ability to make discretionary profitsharing contributions. We made no profit sharing contributions during fiscal years 2000, 1999 or 1998. We also sponsor a Canadian Separation Allowance Plan CSAP ; which provides benefits to substantially all Canadian associates. The CSAP is an unfunded book reserve arrangement; as such, the amounts due to associates are recorded as a liability in the consolidated balance sheets of the Company. CSAP expense for fiscal years 2000, 1999 and 1998 amounted to 2, 000, 7, 000 and 3, 000, respectively.
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Candidiasis is among the most common conditions in people with HIV. While candidiasis is a relatively common condition in the general population, it is often the first illness and signal that HIV disease is progressing to a more severe stage, particularly infections that are recurrent or less responsive to treatment. Candidiasis outbreaks can be frequent, can cause great discomfort and can add to the decline in health seen in AIDS. Preventing and treating oral candidiasis, like other forms of the infection, is important not only because it decreases discomfort caused by the condition, but it also prevents further damage to the immune system. For information on vaginal or systemic candidiasis, read Project Inform's publications, Vaginal Candidiasis and Systemic Candidiasis, available at 1-800-822-7422 or projectingform.
Total RNA was isolated from smooth muscle cells using a commercially available kit RNAzol, Biotecx Laboratories Inc ; , quantified by absorbance at 260 nm, and stored at -70C in a mercaptoethanol ethanol ammonium acetate solution. A unique 40-mer oligonucleotide probe ; for the 70 kD subunit 0, ; of the rat lung soluble guanylate cyclase sGC ; was synthesized on an automated DNA synthesizer using phosphoramidite chemistry based on the sequence published by Nakane et al.34 The probe was labeled with [a-32P]deoxycytidine 5'-triphosphate NEN Research Products ; using a random primer labeling kit Pharmacia LKB and echinacea.
10: 30AM GJ.00001 The Diffusion Fish , THOMAS PEACOCK, ROMAN STOCKER, ERIC LAUGA, CHIANG MEI, MIT -- Nature has evolved a prodigious variety of propulsion mechanisms. We have discovered a remarkable new one that requires no moving parts, instead coupling molecular diffusion and gravity to spontaneously propel asymmetric objects in stratified fluids. This can occur in lakes or marine environments, where temperature and salinity often create stable density gradients. The phenomenon is demonstrated using a triangular wedge, "the diffusion fish, " in salt-stratified water. 10: 43AM GJ.00002 Diffusion-Driven Layering1 , MICHAEL ALLSHOUSE, TOM PEACOCK, Massachusetts Institute of Technology -- Diffusion-driven flow arises in stratified fluids with inclined boundaries. We have discovered that when this phenomenon occurs on the surface of a concentration of neutrally-buoyant particles, the diffusion evolution of the system is significantly altered; providing a novel mechanism for generating thin density layers. We present a series of complementary analytical and experimental studies that clearly demonstrate the effect.
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Research articles Slobodan B. Mickovski & A. Roland Ennos: Anchorage and asymmetry in the root system of Pinus peuce. 161173 Mikhail V. Kozlov & Pekka Niemel: Drought is more stressful for northern populations of Scots pine than low summer temperatures. 175180 Jarkko Hantula & Eeva Vainio: Specific primers for the differentiation of Heterobasidion annosum s r. ; and H. parviporum infected stumps in northern Europe. 181187 Jiao-jun Zhu, Yutaka Gonda, Takeshi Matsuzaki & Masashi Yamamoto: Modeling relative wind speed by optical stratification porosity within the canopy of a coastal protective forest at different stem densities. 189204 Janne Levula, Hannu Ilvesniemi & Carl Johan Westman: Relation between soil properties and tree species composition in a Scots pineNorway spruce stand in southern Finland. 205218 Jyrki Hytnen: Effects of wood, peat and coal ash fertilization on Scots pine foliar nutrient concentrations and growth on afforested former agricultural peat soils. 219234 Sylvain Jutras, Hannu Hkk, Virpi Alenius & Hannu Salminen: Modeling mortality of individual trees in drained peatland sites in Finland. 235251 Jari Krn, Eric Hansen & Heikki Juslin: Environmental activity and forest certification in marketing of forest products a case study in Europe. 253267 Teijo Palander, Katja Turunen & Sanna Laukkanen: Attitude of Finnish timber buyers towards implementation of a forest computer visualisation. 269281 Tysk Staffan Ericsson, Lars stlund & Rikard Andersson: Destroying a path to the past the loss of culturally scarred trees and change in forest structure along Allmunvgen, in mid-west boreal Sweden. 283298 and eletriptan.
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