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HAYFEVER I have always suffered from hay fever for as long as I can remember. Every summer, tasks such as cutting the lawns were quite a chore with almost more time spent sneezing and wiping a running nose with a handkerchief than actually cutting the lawns! I used to take one of the many pharmacy drugs available to help alleviate the symptoms, but they only partially reduced the symptoms and tended to cause side effects like a bleeding nose. I have now been taking transfer factors for a year and can report that I no longer suffer from hay fever. Grant Harrington.
Regional Sympathetic Effects of Low-Dose Clonidine in Heart Failure Anuradha Aggarwal, Murray D. Esler, Margaret J. Morris, Gavin Lambert and David M. Kaye Hypertension 2003; 41; 553-557; originally published online Feb 24, 2003; DOI: 10.1161 01.HYP.0000055779.93635.A2.
Sergei Turovets1 is a Computational Physicist at NeuroInformatics Center, University of Oregon. His current research interests include mathematical modelling and numerical methods in neuroscience and brain imaging, in particular, in electrical impedance and diffuse optical tomography. Dr Allen D Malony1 is a Professor in the Department of Computer & Information Science and the Director of the Performance Research Laboratory at the University of Oregon. His research interests are in parallel computing, performance analysis, supercomputing, and scientific software environments. Dr Pieter Poolman2 is a Scientist at Electrical Geodesics, Inc. in Eugene, Oregon. His research interests include forward and inverse modeling, functional brain imaging, and signal processing. In addition, he is also interested in real-time human performance measurements.
No. of Male Children, 12-17 Years 02 * 02 90XYOne Two Three or more None 02 * 05 90XY.
Each "wave" includes a data collection instrument for collecting "core" data, that will allow collection of a consistent set of fundamental data for research questions that requre a large sample size. Wave 2 includes a "noncore" component designed to address additional research questions that require smaller sample sizes. Type Length Comment char 1 D2Q D3: Does not know how to drive char 1 D2Q D3: Must be trans on stretcher char 1 D2Q D3: No access to a car char 1 D2Q D3: Not able to drive a car char 1 D2Q D3: Oth reason pt. Can't drive char 1 D2Q D3: Requires help walking climb char 1 D2Q D3: Too weak sick after dial char 40 D2Q D3a: Oth reason for not driving char 1 D2Q D4: Person who helps w trans char 1 D2Q D5: Who pays: Dialysis Unit char 1 D2Q D5: Who pays: Myself family char 1 D2Q D5: Who pays: Public agency char char 1 D2Q D5: Who pays: Other char 1 D2Q D6a: Short trt due to transport char 1 D2Q D6b: Skip trt due to transport num 8 E1: Wage rate char 1 D2Q E1a: Not currently employed num 8 D2Q E2: Est wage rate if not empl char 1 D2Q E3: Work limited due to health char 1 D2Q E4: Work amt limited due to hlth char 1 D2Q F1: Frequency of exercise char 1 D2Q F2: Feelings about qual of self char 1 D2Q F3: Not working: Lose benefits char 1 D2Q F3: Not working: Facility sched. char 1 D2Q F3: Not working: No oth job avail char 1 D2Q F3: Not working: Did not want char 1 D2Q F3: Not working: Needed for oth char 1 D2Q F3: Not working: I retired char 1 D2Q F3: Not working: Too sick char 1 D2Q F3: Not working: Job too tiring char 1 D2Q F3: Not working: Dial trt too char 1 D2Q F4: Desire to return to work char 1 D2Q F5a: Trt interfere w work char 1 D2Q F5b: Trt schedule could chang char 1 D2Q F5c: No trt schedule avail to char 1 D2Q F6: Assist given to compl form char 1 D2Q F7: Who helped complete form num 8 DMMS Patient ID date 8 DW2.M Date of birth calculated char 2 DW2.M : First Dialysis day char 2 DW2.M : First Dialysis Month char 2 DW2.M : First Dialysis year char 1 DW2.M : Modality of Trt H PD char 2 DW2.M : Patients Birth day char 2 DW2.M : Patients Birth Mth char 1 DW2.M : Patients Birth yr char 1 DW2.M : Pt complete Dialysis PtQ.
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Trogen-dependent human breast tumor xenografts. Breast Cancer Res Treat 79: 287299 Thornton JW, Need E, Crews D 2003 Resurrecting the ancestral steroid receptor: ancient origin of estrogen signaling. Science 301: 17141717 Weigel NL, Zhang Y 1998 Ligand-independent activation of steroid hormone receptors. J Mol Med 76: 469479 Zhang Y, Bai W, Allgood VE, Weigel NL 1994 Multiple signaling pathways activate the chicken progesterone receptor. Mol Endocrinol 8: 577584 Mani SK, Allen JM, Lydon JP, Mulac-Jericevic B, Blaustein JD, DeMayo FJ, Conneely O, O'Malley BW 1996 Dopamine requires the unoccupied progesterone receptor to induce sexual behavior in mice. Mol Endocrinol [Erratum 1997 ; 11: 423] 10: Blaustein JD 2004 Minireview: neuronal steroid hormone receptors: they're not just for hormones anymore. Endocrinology 145: 10751081 Jacobsen BM, Richer JK, Schittone SA, Horwitz KB 2002 New human breast cancer cells to study progesterone receptor isoform ratio effects and ligand-independent gene regulation. J Biol Chem 277: 2779327800 Lange CA, Shen T, Horwitz KB 2000 Phosphorylation of human progesterone receptors at serine-294 by mitogenactivated protein kinase signals their degradation by the 26S proteasome. Proc Natl Acad Sci USA 97: 10321037 Rowan BG, Garrison N, Weigel NL, O'Malley BW 2000 8-Bromo-cyclic AMP induces phosphorylation of two sites in SRC-1 that facilitate ligand-independent activation of the chicken progesterone receptor and are critical for functional cooperation between SRC-1 and CREB binding protein. Mol Cell Biol 20: 87208730 Grande MA, van der Kraan I, de Jong L, van Driel R 1997 Nuclear distribution of transcription factors in relation to sites of transcription and RNA polymerase II. J Cell Sci 110: 17811791 Elbi C, Misteli T, Hager GL 2002 Recruitment of dioxin receptor to active transcription sites. Mol Biol Cell 13: 20012015 Lim CS, Baumann CT, Htun H, Xian W, Irie M, Smith CL, Hager GL 1999 Differential localization and activity of the A- and B-forms of the human progesterone receptor using green fluorescent protein chimeras. Mol Endocrinol 13: 366375 Thompson EW, Paik S, Brunner N, Sommers CL, Zugmaier G, Clarke R, Shima TB, Torri J, Donahue S, Lippman ME, Dickson RB 1992 Association of increased basement membrane invasiveness with absence of estrogen receptor and expression of vimentin in human breast cancer cell lines. J Cell Physiol 150: 534544 Sommers CL, Byers SW, Thompson EW, Torri JA, Gelmann EP 1994 Differentiation state and invasiveness of human breast cancer cell lines. Breast Cancer Res Treat 31: 325335 van Belzen N, Dinjens WN, Diesveld MP, Groen NA, van der Made AC, Nozawa Y, Vlietstra R, Trapman J, Bosman FT 1997 A novel gene which is up-regulated during colon epithelial cell differentiation and down-regulated in colorectal neoplasms. Lab Invest 77: 8592 Stetler-Stevenson WG, Aznavoorian S, Liotta LA 1993 Tumor cell interactions with the extracellular matrix during invasion and metastasis. Annu Rev Cell Biol 9: 541573 Bartsch JE, Staren ED, Appert HE 2003 Adhesion and migration of extracellular matrix-stimulated breast cancer. J Surg Res 110: 287294 Davidson NE, Wolff AC 2001 The use of antracyclines and taxanes for adjuvant therapy of breast cancer. Breast 10 Suppl 3 ; : 9095 Coleman RE 2003 Current and future status of adjuvant therapy for breast cancer. Cancer 97: 880886 Pater JL, Pritchard KI 2003 Optimal chemotherapy for women with breast cancer: the plot thickens. J Clin Oncol 21: 963964 and pediatric.
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Glade Lab Move In August 2002 CNS moved the 75-seat Glade lab from the basement of the Steacie Science Library, to the renovated space in the Chemistry Building that was previously occupied by the Ariel lab. Netware cluster for ACADLABS This project established a redundant, reliable service with file access and expansion capability appropriate to the community needs, supporting approximately 500 seats in 8 separate computing facilities and provides application and file storage for 40, 000 accounts.
Compared with the control Table 6 ; . Regardless of the concentrations applied, PBZ-treated trees had lower values for tree volume and length of new shoots compared with the control. During the second-round observation, 6 months after treatment application, there were significantly lower results for PBZ-treated trees than the control trees for all the vegetative parameters considered Table 7 ; . In all instances, rates of PBZ applied had an impact on the parameters and the methods of application did not affect the results. PBZ at a rate of 8.25 g a.i. per tree significantly reduced leaf number and leaf area compared with the control trees and pegasys
Galetti, M. & Aleixo, A. 1998 ; : Effects of palm heart harvesting on avian frugivores in the Atlantic rainforest of Brazil. - Journal of Applied Ecology 35: 286-293.
Stretches of nucleic acids DNA or RNA based ; or chemically modified versions thereof see also Part I, Chapter 8 ; . The nucleotide sequence specificity of these antisense molecules allows them to bind to specific gene or more commonly ; mRNA sequences, thereby preventing gene expression by blocking either transcription or translation [40]. The therapeutic rationale underlining this approach stems from the fact that many diseases are triggered or are exacerbated by inappropriate expression overexpression of specific genes. Antisense, in principle, provides a mechanism by which this can be blocked. While the underlining concept is straightforward, like gene therapy, it is proving more difficult to apply in practice. Major difficulties have arisen in relation to product nuclease sensitivity, product targeting, delivery and cellular uptake. Vitravene fomivirsen sodium, ISIS Pharmaceuticals; see Table 9 ; remains the only antisense-based biopharmaceutical approved for general medical use see also Part III, Chapter 3 ; . The product is a 21base phosphorothioate nucleotide that displays a base sequence complementary to certain human cytomegaloviral mRNA transcripts. Its administration inhibits viral replication through an antisense mechanism. Approved in the US in 1998 and in the EU in 1999, the product is indicated for the treatment of cytomegalovirus retinitis by intraocular injection in AIDS patients. It was withdrawn from the EU market in May 2002 for commercial reasons. RNAi represents an alternative and more recently pursued mechanism of downregulating gene expression [41] and references therein ; see also Part II, Chapter 8 and Part I, Chapter 1 and 10 ; . The RNAi pathway was first discovered in plants, but it is now known to function in most if not all eukaryotes. RNAi repre and pegfilgrastim.
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The aim of this study was to determine the effect of CGP 12177 and the other aryloxypropanolamines, bupranolol and SR 59230A, on the contraction of rat pulmonary artery and the possible interaction of these ligands with 1-AR. CGP 12177 elicited a relaxation in PHE-precontracted arteries, had negligible effect when applied on basal tone, but further enhanced contraction and intracellular calcium concentration in PGF2-precontracted preparations. CGP 12177-induced contraction was inhibited by phentolamine and PBZ -AR antagonists ; and also by bupranolol and SR 59230A. Moreover, CGP 12177, bupranolol and SR 59230A antagonized PHE-induced contraction. Finally, it is shown that CGP 12177, bupranolol and SR 59230A exhibited affinity for [3H]-prazosin binding sites. In this study, CGP 12177 induced relaxation for concentrations 1 M in PHEprecontracted arteries. This is consistent with the potency of CGP 12177 in other rat arteries Trochu et al., 1999; Brahmadevara et al., 2003; Kozlowska et al., 2003 ; . Recent reports highlight the influence of the precontractile agonist on the amplitude of the relaxant effect of some -AR agonists. For instance, CGP 12177 produced relaxation in PHE-precontracted rat aorta, but failed to do so PGF2-precontracted one Brahmadevara et al., 2003 ; . Similarly, the relaxation evoked by cyanopindolol another aryloxypropanolamine, non-conventional partial agonist ; in rat mesenteric artery was strongly reduced in vessels constricted with PGF2, compared to those precontracted with PHE Kozlowska et al., 2003 ; . In the present study, the effect of CGP 12177 was also evaluated in arteries precontracted with PGF2 instead of PHE. Surprisingly, in these arteries, CGP 12177 exerted no relaxant effect, but rather induced contraction. These two opposite effects contraction or relaxation ; were observed within the same range of concentrations, suggesting a common underlying mechanism. To our knowledge, it is the first time that a contractile effect of CGP 12177 is reported. The use of relatively low concentrations of PGF2 producing only 15 % of its maximum response ; might explain why contractile effects of CGP 12177 could be unmasked here. Accordingly, increasing pre-contraction level resulted in a marked reduction in the amplitude of CGP 12177-induced contraction. Interference of CGP 12177 with -AR or -AR-mediated intracellular signaling pathway has been postulated from experiments showing that CGP 12177 produced relaxation in PHE!
To determine the optimal time delay we use the phase space analog of the autocorrelation function, an information-theoretic concept, the average mutual information AMI ; . The optimal delay for the phase space reconstruction is chosen at its first minimum. The product of this delay min with the value of I min on sums smaller than the data set length is singularly useful when used in a moving average manner to test for internal consistency of subsets of the data. The autocorrelation function for these data itself has a shallow flat minimum from 8 to 12 days whereas the AMI delay was 7 days for the historical 55 000 point data set. Since the reliability of the choice increases with length of data set the value of 7 was used as a reference. The analyses were, however, performed for a range of delays: 3, 7, 11, and 101 days. Though the curves presented are for t 7 days, for all the delays considered, including the range defined by the broad flat minimum of the autocorraletion function, the reported behavior was observed. The optimal dimension of the phase space is obtained using the minimum of false nearest neighbors. However, the embedding dimension is hard to determine reliably on short noisy data sets. Thus, we use it only to set a lower bound in terms of the smallest dimension in which to expect the signature of potential chaotic behavior in the correlation sum and local dimension plots and pegvisomant.
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Chapter 1. Introduction surface, which stabilizes the folded state relative to the unfolded state because it exposes less surface area from which the co-solute must be expelled Arakawa and Timasheff, 1982 ; . Furthermore, cryoprotectants stabilize dehydrated proteins due to substitution of essential water molecules by formation of hydrogen bonds Prestrelski et al., 1993a and b ; . Amino acid addition can result in stabilization due to chelation of metal ions, antioxidant activity, aggregation inhibition or preferential exclusion Shiraki et al., 2004 and Valente et al., 2005 ; . Divalent metal ions, such as calcium, were reported to stabilize certain enzymes. However, calcium and magnesium ions were reported to destabilize lysozyme Wang, 1999 ; . Some proteins can be stabilized by the addition of zinc ions, which was attributed to a decreased susceptibility to degradation due to a lowered solubility of so formed physically crosslinked protein oligo- or polymers e.g., insulin, recombinant human growth hormone, recombinant human nerve growth factor and bovine serum albumin ; Johnson et al., 1997b, Costantino et al., 2000 and Fu et al., 2000a ; . The dynamics of the zinc-complexation are determined by the affinity of the protein for the metal, availability of metal coordination sites, protein and metal concentrations, and solution pH Yang et al., 2000a ; . Consequently, the re-dissolution of the complexes can be sensitive to variations in the ionic environment Prabhu et al., 2001 ; . Although lysozyme has the ability to bind 2 mol of zinc- or copper II ; -ions Moreau 1995 ; , no physical stabilization by such a electrostatic crosslinking was reported for both ions. Copper-ions contributed to an improved thermal stability of dissolved lysozyme due to a suppression of disulfide interchange Tomizawa et al., 1995, Putney and Burke, 1998 ; . However, metal ion binding could also affect the structural integrity negatively. Copperbinding, for example, was discussed to cause the irreversible denaturation of the native cellular prion protein into the insoluble and protease-resistant pathologic form in CreutzfeldtJakob disease Quaglio et al., 2001 ; . Binding of substrates, inhibitors and cofactors could lead to a stabilization of proteins. Tri-Nacetylglucosamine, for example, increased the molecular rigidity of the protein conformation by binding at the active site of lysozyme Pace and McGrath, 1980 ; . Surfactants are added to protein solutions in a concentration close to the critical micelle concentration to decrease the surface tension and compete with the protein for adsorption at the surface or interface Wang, 1999 ; . Non-ionic polysorbate and poloxamer 188 are often used but also anionic SDS can be found in a lyophilized drug product Proleukin, Chiron, US ; . Surfactants also bind to proteins, which could lead to solubilization or destabilize the native state of the protein or to inhibition paragraph 1.1.2.2.2. ; . Furthermore, it was reported 21.
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Application . 48 Table 4.1 Effect of PBZ on leaf, stem and root characteristics. Mean value standard deviation. Table 5.1 Chlorophyll a and chlorophyll b, stomatal conductance Gs ; , rate of transpiration E ; , net photosynthesis Pn ; of leaf tissue and potato plant height as influenced by rates of PBZ application . Table 5.2 Days to physiological maturity for potato plants grown in a hot tropical lowland as influenced by PBZ application method and rate. Table 5.3 Total dry matter production g ; and distribution % ; amongst different parts of potato plants grown under a hot tropical condition, as influenced by rate and method of PBZ application . Table 6.1 Partitioning coefficient PC ; of potato as influenced by different rates of PBZ. Table 7.1 Days to tuber initiation, fresh mass, number, dry matter content, and specific gravity of potato tubers as affected by rates of PBZ. 98 Table 7.2 The effect of application method and rate of PBZ on the crude protein content and dormancy period of potato. 99 Table 7.3 Potassium, calcium, magnesium, sulphur, copper and zinc concentrations dry matter basis ; in potato tubers as affected by application method and concentration of PBZ. 100 89 76 and pemetrexed.
The greenhouse and field trials demonstrated that MCPA and PBZ effectively prevented flower formation and berry set in potato without negatively affecting tuber yield and quality. Application of the two chemicals during the early flower bud stage gave slightly higher tuber yields than late application. The trials were conducted using only one cultivar and under sub.
EXPRESSION OF AQP1, AQP3 AND AQP5 IN THE HUMAN LUNG CANCER TISSUE Makoto Sugita MD * Sumiko Maeda MD Motoyasu Sagawa MD Miyako Shimasaki MS Yoshimichi Ueda MD Tsutomu Sakuma MD Kanazawa Medical University, Ishikawa, Japan PURPOSE: The movement of water across cell membrane is essential to life. Recent investigations have demonstrated that aquaporins AQPs ; , a family of water channels, are essential for maintaining the transmembrane water transport. Among at least 11 AQPs identified in mammalian tissues, AQP1, AQP3, AQP4, and AQP5 have been recognized in the lung, however, their expression and functions in the normal and diseased human lungs are poorly understood. In this study, we aimed to investigate the expression of AQPs in the human lung cancer tissue. We examined and pemoline.
Alocorticoid effects 76 79 ; , and it and or a metabolite are likely culprits in this phenomenon. However, there is as yet no evidence that 17-OHP has direct or indirect antimineralocorticoid effects. Salt wasting may include such nonspecific symptoms as poor appetite, vomiting, lethargy, and failure to gain weight. Severely affected patients with CAH usually present at 1 4 weeks of age with hyponatremia, hyperkalemia, hyperreninemia see Section IV.C.2 ; and hypovolemic shock. These "adrenal crises" may prove fatal if proper medical care is not delivered. This problem is particularly critical in infant boys who have no genital ambiguity to alert physicians to the diagnosis of CAH before the onset of dehydration and shock 80 ; . The mortality rate for CAH remains high in such patients, as suggested by the relative paucity of male patients identified in case reports 81 ; . It for this reason that many states in the United States and a number of countries have adopted newborn screening for CAH see Section IV.B ; . The rapidity of onset and severity of a salt wasting crisis may reflect the individual's ancillary homeostatic mechanisms for sodium and fluid conservation. Such factors might include the concentration and transcriptional activity of mineralocorticoid receptors in the kidney and elsewhere, and the ability to increase vasopressin or decrease atrial natriuretic factor 82 ; in response to volume contraction. Siblings may be discordant for salt wasting 83 ; . Furthermore, CAH patients known to have severe salt wasting episodes in infancy and early childhood may show improved sodium balance and relatively more efficient aldosterone synthesis with age. Unrelated individuals carrying identical mutations may manifest different degrees of salt wasting 84 ; . Although explanations for these observations are not immediately apparent, both genetic and nongenetic factors may contribute to the presence or absence of the salt wasting trait. Extraadrenal 21-hydroxylase has been detected by in vivo metabolic studies 85 ; , but molecular genetic investigation has yielded contradictory results as to whether CYP21 could be a source for this activity 86 89 ; . Other enzymes with 21-hydroxylase activity have not been identified in humans, although such enzymes have been identified in rabbit liver 90 and pbz.
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No one familiar with the study's authors would be surprised by their conclusions. Olshansky tops the list of the nation's life-expectancy naysayers. Dr. Richard Suzman, associate director of the Behavioral and Social Research Program and penicillamine.
The treatments were basal drench or soil injection with pbz formulated as profile 2sc or water as a control in complete random experimental designs.
Nisms underlying the etiopathogenesis of fibromyalgia remain elusive. Some psychiatric studies suggest a relationship to anxiety disorder in a significant subgroup [1, 2], while other studies have found that fibromyalgia is highly associated with depressive disorders [37]. Many psychometric studies indicate an association between fibromyalgia, chronic distress, anxiety, depression and certain personality traits [8 15], although conflicting results exist [16, 17]. During the last decade many authors have suggested that perturbations in the autonomic nervous system is a key element in the fibromyalgia syndrome, however, contradictory results have been reported [1823]. Also perturbations of the hypothalamic-pituitary-adrenal HPA ; axis, leading to relative insufficiency of the adrenal glands and hypocortisolism, is a neuroendocrine finding that has been described in fibromyalgia patients [2426]. Stress, either physical, emotional or metabolic in nature, may be the essential underlying factor leading to psychiatric and somatic symptoms in fibromyalgia patients through a final common pathway [27]. The physiology of stress includes CNS and peripheral components, involving the hypothalamic-pituitary-adrenal HPA ; axis, the hypothalamic-pituitary-gonadal HPG ; axis and the autonomic sympathetic ; system.[28, 29]. Stress, leading to perturbations in all these systems, may elicit pain in fibromyalgia patients [30]. Although a vast literature concerning etiopathogenetic factors in the fibromyalgia syndrome exists, we have found no published studies focusing upon which of all these factors that are most important to explain the ever changing and troublesome pain in these patients. This is a clinical important and meaningful question, as knowledge about factors influencing variance in pain is essential in the search for more efficacious pain treatment for fibromyalgia patients. To clarify this question, we assessed pain, psychiatric symptoms and personality factors in patients with fibromyalgia and population based controls. We also used a psychopharmacological challenge test to assess autonomic and adrenocortical cortisol responsiveness. We hypothesized that a substantial proportion of the subjectively experienced variance in pain in fibromyalgia patients would be explained by psychological factors alone, but that a combined model also including neuroendocrine and autonomic factors as a base of the symptomatology would give the most likely explanation of variance in pain and pennyroyal.
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Mean standard error. N number of animals. SS steady state. E experimental value five minutes after procedure hyperventilation ; . D difference between SS and E. Statistical significance of D P statistical significance of the difference between D before PBZ and D after PBZ infusion. CBF -- cerebral blood flow ml 100 gm brain min ; . CVR cerebral vascular resistance mm Hg ml 100 gm brain min ; . Paco 2 arterial carbon dioxide tension mm Hg ; . CPP cerebral perfusion pressure mm Hg ; . C.I. chemical index ml 100 gm brain min mm Hg ; . ICVP intracranial venous pressure and pediatric.
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